BPC-157 is a synthetic pentadecapeptide — a chain of 15 amino acids — with the sequence Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val, derived from a partial sequence of a protein found in gastric juice. This page is a chemistry and handling reference for the compound.
Identity and physical data
| Property | Value |
|---|---|
| Class | Synthetic pentadecapeptide (15 residues) |
| Sequence (one-letter) | GEPPPGKPADDAGLV |
| Sequence (three-letter) | Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val |
| Molecular formula | C62H98N16O22 |
| Molecular weight | 1419.5 g/mol (free base) |
| CAS number | 137525-51-0 |
| Appearance | White to off-white lyophilized powder |
| Solubility | Water soluble |
| Also referred to as | Body Protection Compound-157, PL 14736, Pentadecapeptide BPC 157 |
Structural notes
Three features of the sequence are worth knowing when handling the material:
- The proline triplet (positions 3-5). Consecutive prolines constrain the backbone conformation and contribute to the peptide's characteristic stability profile relative to many peptides of similar length.
- Two aspartate residues (positions 10-11). Adjacent Asp residues are a known site for pH-dependent degradation chemistry in peptides, which is one reason strongly acidic storage conditions are avoided.
- No cysteine, no disulfide bonds. There is no oxidation-sensitive thiol in the sequence, so the compound does not require the reducing-agent precautions that cysteine-containing peptides do.
Free base vs acetate salt
Synthetic peptides are typically isolated as a salt of the counter-ion used during purification — most often acetate, sometimes trifluoroacetate (TFA). The molecular weight above is for the free base. A vial of acetate salt weighs more per mole of peptide than the free base figure implies, which is one of the contributors to the gap between gross vial weight and actual peptide mass. This is covered in net peptide content vs gross weight. A Certificate of Analysis should state the salt form.
Handling and storage
- Dry powder: store at −20 °C, sealed, protected from light and moisture. Lyophilized material is stable long-term under these conditions.
- Before opening: equilibrate the sealed vial to room temperature so moisture does not condense onto cold powder.
- Reconstitution: bacteriostatic water is the standard solvent. Add solvent slowly against the vial wall rather than directly onto the powder cake, and swirl rather than shake — mechanical agitation and foaming are avoidable sources of degradation.
- In solution: refrigerate at 2-8 °C, protect from light, and avoid repeated freeze-thaw cycles. For longer storage of a working solution, aliquot into single-use volumes before freezing.
Full procedure: reconstituting lyophilized peptides. Stability detail: peptide storage and stability.
What to verify on the Certificate of Analysis
- Mass spectrometry: observed mass should agree with the theoretical 1419.5 g/mol for the free base, within the method's stated tolerance.
- HPLC purity: reported as area percentage, with the detection wavelength stated.
- Salt form and net peptide content: acetate or TFA, and the peptide fraction of total powder mass.
- Batch identifier and analysis date matching the vial you received.
Deletion sequences — chains missing one residue — are the most common synthesis-related impurity in a peptide of this length and appear as shoulder peaks close to the main peak on the chromatogram. See how to read a COA.
Availability
BPC-157 100 mg is supplied as lyophilized powder in a sealed vial with batch documentation published on the product page. Bacteriostatic water is sold separately. Related compounds in the catalog include TB-500 (Thymosin Beta-4 fragment) and KPV.
Intended for laboratory and research use only. Not a medicine. Not for human or veterinary use, and not for diagnostic or therapeutic application. No claims are made regarding safety or efficacy in humans.